Methylene blue ignites your mitochondria

Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways

Hani Atamna1, Andy Nguyen, Carla Schultz, Kathleen Boyle, Justin Newberry, Hiroyuki Kato and Bruce N. Ames Nutrition and Metabolism Center, Children's Hospital Oakland Research Institute, Oakland, California, USA

Methylene blue (MB) has been used clinically for about a century to treat numerous ailments. We show that MB and other diaminophenothiazines extend the life span of human IMR90 fibroblasts in tissue culture by >20 population doubling (PDLs).
MB delays senescence at nM levels in IMR90 by enhancing mitochondrial function. MB increases mitochondrial complex IV by 30%, enhances cellular oxygen consumption by 37–70%, increases heme synthesis, and reverses premature senescence caused by H2O2 or cadmium.

MB also induces phase-2 antioxidant enzymes in hepG2 cells. Flavin-dependent enzymes are known to use NAD(P)H to reduce MB to leucomethylene blue (MBH2), whereas cytochrome c reoxidizes MBH2 to MB. Experiments on lysates from rat liver mitochondria suggest the ratio MB/cytochrome c is important for the protective actions of MB.

We propose that the cellular senescence delay caused by MB is due to cycling between MB and MBH2 in mitochondria, which may partly explain the increase in specific mitochondrial activities. Cycling of MB between oxidized and reduced forms may block oxidant production by mitochondria. Mitochondrial dysfunction and oxidative stress are thought to be key aberrations that lead to cellular senescence and aging. MB may be useful to delay mitochondrial dysfunction with aging and the decrease in complex IV in Alzheimer disease.

—Atamna, H., Nguyen, A., Schultz, C., Boyle, K., Newberry, J., Kato, H., Ames, B. N. Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways.

More info: https://wiki.brown.edu/confluence/download/attachments/24806537/methylene+blue.pdf

Also: Methylene blue and Alzheimer's disease
Potential Alzheimer's, Parkinson's Cure Found In Century-old Drug

Warning:
Methylene blue is a monoamine oxidase inhibitor (MAOI),[5] and if infused intravenously at doses exceeding 5 mg/kg, may precipitate serious serotonin toxicity, serotonin syndrome, if combined with any selective serotonin reuptake inhibitors (SSRIs) or other serotonin reuptake inhibitor (e.g., duloxetine, sibutramine, venlafaxine, clomipramine, imipramine).[6]

Methylene blue is also structurally similar to the chlorpromazine and the typical antipsychotics. It is the basic compound from which chlorpromazine and many other antipsychotics are made.[7]

Source: http://en.wikipedia.org/wiki/Methylene_blue

Het verband tussen mazelen, gluten en autisme

http://www.europeanlaboratory.nl/documents/autisme.pdf


Het enzym DPPIV verantwoordelijk voor de afbraak van opioïden zoals gluten, melk (caseïne), soya en spinazie is ook een belangrijke factor bij autisme.
Uit onderzoek blijkt dat deze mensen ook een verhoogde concentratie giftige opioïden (= exorfinen) in de hersenen opgestapeld hebben omdat ze het onvoldoende kunnen afbreken in de darmen. Een lekke darm is een rechtstreeks gevolg van dit mankement.

Voorbeelden van verbetering met een exorfinenvrij dieet vind je hieronder:
http://people.emich.edu/jtodd/whiteley_et_al_1999.pdf


"ME ziekte is blijkbaar nog niet ernstig genoeg in de ogen van politici,... wij zien dat heel anders!" door Catryn V